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Mouse thymoma EL4/13.18 library (LMBP LIB15)
| Library name: | Mouse thymoma EL4/13.18 library |
| Library type: | cDNA |
| Library accession number: | LMBP LIB15 |
| Organism: | Mus musculus |
| Priming method: | Oligo(dT) + Random (bidirectional) |
| Vector name: | pCAGGS |
| Vector sequence file: | p2453.txt |
| Vector circular map: | p2453.pdf |
| Vector accession number: | LMBP 2453 |
| Vector type: | eukaryotic expression vector |
| Cloning sites: | EcoRI |
| Library coverage: | 1,3 x 10E6 |
| Average insert size: | 1,55 kb |
| Distributed as: | pooled library DNA |
| DNA concentration: | 150 ng/µl |
| Volume: | 5 µl |
| Further information: | The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones. |
| History of deposit: | The cDNA library was deposited by Prof. R. Contreras1. 1 Department of Molecular Biology, Ghent University, Belgium |
| Library reference: | - [PMID: -] |
| Related reference: | - |
| Restricted distribution: | - BCCM MTA |
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